Receptor-Like Kinases BAK1 and SOBIR1 Are Required for Necrotizing Activity of a Novel Group of Sclerotinia sclerotiorum Necrosis-Inducing Effectors.
Identifieur interne : 000065 ( Main/Exploration ); précédent : 000064; suivant : 000066Receptor-Like Kinases BAK1 and SOBIR1 Are Required for Necrotizing Activity of a Novel Group of Sclerotinia sclerotiorum Necrosis-Inducing Effectors.
Auteurs : Shirin Seifbarghi [Canada] ; Mohammad Hossein Borhan [Canada] ; Yangdou Wei [Canada] ; Lisong Ma [Canada] ; Cathy Coutu [Canada] ; Diana Bekkaoui [Canada] ; Dwayne D. Hegedus [Canada]Source :
- Frontiers in plant science [ 1664-462X ] ; 2020.
Abstract
Sclerotinia sclerotiorum is a characteristic necrotrophic plant pathogen and is dependent on the induction of host cell death for nutrient acquisition. To identify necrosis-inducing effectors, the genome of S. sclerotiorum was scanned for genes encoding small, secreted, cysteine-rich proteins. These potential effectors were tested for their ability to induce necrosis in Nicotiana benthamiana via Agrobacterium-mediated expression and for cellular localization in host cells. Six novel proteins were discovered, of which all but one required a signal peptide for export to the apoplast for necrotizing activity. Virus-induced gene silencing revealed that the five necrosis-inducing effectors with a requirement for secretion also required the plant co-receptor-like kinases Brassinosteroid Insensitive 1-Associated Receptor Kinase 1 (BAK1) and Suppressor of BAK1-Interacting Receptor-like Kinase 1 (SOBIR1) for the induction of necrosis. S. sclerotiorum necrosis-inducing effector 2 (SsNE2) represented a new class of necrosis-inducing proteins as orthologs were identified in several other phytopathogenic fungi that were also capable of inducing necrosis. Substitution of conserved cysteine residues with alanine reduced, but did not abolish, the necrotizing activity of SsNE2 and full-length protein was required for function as peptides spanning the entire protein were unable to induce necrosis. These results illustrate the importance of necrosis-inducing effectors for S. sclerotiorum virulence and the role of host extracellular receptor(s) in effector-triggered susceptibility to this pathogen.
DOI: 10.3389/fpls.2020.01021
PubMed: 32754179
PubMed Central: PMC7367142
Affiliations:
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Hegedus</name><affiliation wicri:level="1"><nlm:affiliation>Agriculture and Agri-Food Canada, Saskatoon, SK, Canada.</nlm:affiliation><country xml:lang="fr">Canada</country><wicri:regionArea>Agriculture and Agri-Food Canada, Saskatoon, SK</wicri:regionArea><wicri:noRegion>SK</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:affiliation>Department of Food and Bioproduct Sciences, University of Saskatchewan, Saskatoon, SK, Canada.</nlm:affiliation><country xml:lang="fr">Canada</country><wicri:regionArea>Department of Food and Bioproduct Sciences, University of Saskatchewan, Saskatoon, SK</wicri:regionArea><wicri:noRegion>SK</wicri:noRegion></affiliation></author></analytic><series><title level="j">Frontiers in plant science</title><idno type="ISSN">1664-462X</idno><imprint><date when="2020" type="published">2020</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><i>Sclerotinia sclerotiorum</i> is a characteristic necrotrophic plant pathogen and is dependent on the induction of host cell death for nutrient acquisition. To identify necrosis-inducing effectors, the genome of <i>S. sclerotiorum</i> was scanned for genes encoding small, secreted, cysteine-rich proteins. These potential effectors were tested for their ability to induce necrosis in <i>Nicotiana benthamiana via Agrobacterium</i>-mediated expression and for cellular localization in host cells. Six novel proteins were discovered, of which all but one required a signal peptide for export to the apoplast for necrotizing activity. Virus-induced gene silencing revealed that the five necrosis-inducing effectors with a requirement for secretion also required the plant co-receptor-like kinases Brassinosteroid Insensitive 1-Associated Receptor Kinase 1 (BAK1) and Suppressor of BAK1-Interacting Receptor-like Kinase 1 (SOBIR1) for the induction of necrosis. <i>S. sclerotiorum</i> necrosis-inducing effector 2 (SsNE2) represented a new class of necrosis-inducing proteins as orthologs were identified in several other phytopathogenic fungi that were also capable of inducing necrosis. Substitution of conserved cysteine residues with alanine reduced, but did not abolish, the necrotizing activity of SsNE2 and full-length protein was required for function as peptides spanning the entire protein were unable to induce necrosis. These results illustrate the importance of necrosis-inducing effectors for <i>S. sclerotiorum</i> virulence and the role of host extracellular receptor(s) in effector-triggered susceptibility to this pathogen.</div></front></TEI><pubmed><MedlineCitation Status="PubMed-not-MEDLINE" Owner="NLM"><PMID Version="1">32754179</PMID><DateRevised><Year>2020</Year><Month>09</Month><Day>28</Day></DateRevised><Article PubModel="Electronic-eCollection"><Journal><ISSN IssnType="Print">1664-462X</ISSN><JournalIssue CitedMedium="Print"><Volume>11</Volume><PubDate><Year>2020</Year></PubDate></JournalIssue><Title>Frontiers in plant science</Title><ISOAbbreviation>Front Plant Sci</ISOAbbreviation></Journal><ArticleTitle>Receptor-Like Kinases BAK1 and SOBIR1 Are Required for Necrotizing Activity of a Novel Group of <i>Sclerotinia sclerotiorum</i> Necrosis-Inducing Effectors.</ArticleTitle><Pagination><MedlinePgn>1021</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.3389/fpls.2020.01021</ELocationID><Abstract><AbstractText><i>Sclerotinia sclerotiorum</i> is a characteristic necrotrophic plant pathogen and is dependent on the induction of host cell death for nutrient acquisition. To identify necrosis-inducing effectors, the genome of <i>S. sclerotiorum</i> was scanned for genes encoding small, secreted, cysteine-rich proteins. These potential effectors were tested for their ability to induce necrosis in <i>Nicotiana benthamiana via Agrobacterium</i>-mediated expression and for cellular localization in host cells. Six novel proteins were discovered, of which all but one required a signal peptide for export to the apoplast for necrotizing activity. Virus-induced gene silencing revealed that the five necrosis-inducing effectors with a requirement for secretion also required the plant co-receptor-like kinases Brassinosteroid Insensitive 1-Associated Receptor Kinase 1 (BAK1) and Suppressor of BAK1-Interacting Receptor-like Kinase 1 (SOBIR1) for the induction of necrosis. <i>S. sclerotiorum</i> necrosis-inducing effector 2 (SsNE2) represented a new class of necrosis-inducing proteins as orthologs were identified in several other phytopathogenic fungi that were also capable of inducing necrosis. Substitution of conserved cysteine residues with alanine reduced, but did not abolish, the necrotizing activity of SsNE2 and full-length protein was required for function as peptides spanning the entire protein were unable to induce necrosis. These results illustrate the importance of necrosis-inducing effectors for <i>S. sclerotiorum</i> virulence and the role of host extracellular receptor(s) in effector-triggered susceptibility to this pathogen.</AbstractText><CopyrightInformation>Copyright © 2020 Seifbarghi, Borhan, Wei, Ma, Coutu, Bekkaoui and Hegedus.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Seifbarghi</LastName><ForeName>Shirin</ForeName><Initials>S</Initials><AffiliationInfo><Affiliation>Agriculture and Agri-Food Canada, Saskatoon, SK, Canada.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Department of Biology, University of Saskatchewan, Saskatoon, SK, Canada.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Borhan</LastName><ForeName>Mohammad Hossein</ForeName><Initials>MH</Initials><AffiliationInfo><Affiliation>Agriculture and Agri-Food Canada, Saskatoon, SK, Canada.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Wei</LastName><ForeName>Yangdou</ForeName><Initials>Y</Initials><AffiliationInfo><Affiliation>Department of Biology, University of Saskatchewan, Saskatoon, SK, Canada.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Ma</LastName><ForeName>Lisong</ForeName><Initials>L</Initials><AffiliationInfo><Affiliation>Agriculture and Agri-Food Canada, Saskatoon, SK, Canada.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Coutu</LastName><ForeName>Cathy</ForeName><Initials>C</Initials><AffiliationInfo><Affiliation>Agriculture and Agri-Food Canada, Saskatoon, SK, Canada.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Bekkaoui</LastName><ForeName>Diana</ForeName><Initials>D</Initials><AffiliationInfo><Affiliation>Agriculture and Agri-Food Canada, Saskatoon, SK, Canada.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Hegedus</LastName><ForeName>Dwayne D</ForeName><Initials>DD</Initials><AffiliationInfo><Affiliation>Agriculture and Agri-Food Canada, Saskatoon, SK, Canada.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Department of Food and Bioproduct Sciences, University of Saskatchewan, Saskatoon, SK, Canada.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2020</Year><Month>07</Month><Day>10</Day></ArticleDate></Article><MedlineJournalInfo><Country>Switzerland</Country><MedlineTA>Front Plant Sci</MedlineTA><NlmUniqueID>101568200</NlmUniqueID><ISSNLinking>1664-462X</ISSNLinking></MedlineJournalInfo><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">Sclerotinia sclerotiorum</Keyword><Keyword MajorTopicYN="N">cell death</Keyword><Keyword MajorTopicYN="N">necrosis</Keyword><Keyword MajorTopicYN="N">receptors</Keyword><Keyword MajorTopicYN="N">virus-induced gene silencing</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2020</Year><Month>01</Month><Day>15</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2020</Year><Month>06</Month><Day>22</Day></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2020</Year><Month>8</Month><Day>6</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2020</Year><Month>8</Month><Day>6</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2020</Year><Month>8</Month><Day>6</Day><Hour>6</Hour><Minute>1</Minute></PubMedPubDate></History><PublicationStatus>epublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">32754179</ArticleId><ArticleId IdType="doi">10.3389/fpls.2020.01021</ArticleId><ArticleId IdType="pmc">PMC7367142</ArticleId></ArticleIdList><ReferenceList><Reference><Citation>Annu Rev Phytopathol. 2016 Aug 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name="Canada"><noRegion><name sortKey="Seifbarghi, Shirin" sort="Seifbarghi, Shirin" uniqKey="Seifbarghi S" first="Shirin" last="Seifbarghi">Shirin Seifbarghi</name></noRegion><name sortKey="Bekkaoui, Diana" sort="Bekkaoui, Diana" uniqKey="Bekkaoui D" first="Diana" last="Bekkaoui">Diana Bekkaoui</name><name sortKey="Borhan, Mohammad Hossein" sort="Borhan, Mohammad Hossein" uniqKey="Borhan M" first="Mohammad Hossein" last="Borhan">Mohammad Hossein Borhan</name><name sortKey="Coutu, Cathy" sort="Coutu, Cathy" uniqKey="Coutu C" first="Cathy" last="Coutu">Cathy Coutu</name><name sortKey="Hegedus, Dwayne D" sort="Hegedus, Dwayne D" uniqKey="Hegedus D" first="Dwayne D" last="Hegedus">Dwayne D. Hegedus</name><name sortKey="Hegedus, Dwayne D" sort="Hegedus, Dwayne D" uniqKey="Hegedus D" first="Dwayne D" last="Hegedus">Dwayne D. Hegedus</name><name sortKey="Ma, Lisong" sort="Ma, Lisong" uniqKey="Ma L" first="Lisong" last="Ma">Lisong Ma</name><name sortKey="Seifbarghi, Shirin" sort="Seifbarghi, Shirin" uniqKey="Seifbarghi S" first="Shirin" last="Seifbarghi">Shirin Seifbarghi</name><name sortKey="Wei, Yangdou" sort="Wei, Yangdou" uniqKey="Wei Y" first="Yangdou" last="Wei">Yangdou Wei</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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